This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various morpholog...
Rapid screening of red blood cells for active infection of COVID-19 is presented using a compact and field-portable, 3D-printed shearing digital holographic microscope. Video holograms of thin blood smears are recorded, individual red blood cells are...
IEEE journal of biomedical and health informatics
May 1, 2021
Computer-assisted algorithms have become a mainstay of biomedical applications to improve accuracy and reproducibility of repetitive tasks like manual segmentation and annotation. We propose a novel pipeline for red blood cell detection and counting ...
Journal of the Optical Society of America. A, Optics, image science, and vision
Mar 1, 2021
In dual-wavelength interferometry, the key issue is how to efficiently retrieve the phases at each wavelength using the minimum number of wavelength-multiplexed interferograms. To address this problem, a new dual-wavelength interferogram decoupling m...
SIGNIFICANCE: Digital holographic microscopy (DHM) is a promising technique for the study of semitransparent biological specimen such as red blood cells (RBCs). It is important and meaningful to detect and count biological cells at the single cell le...
BACKGROUND: Measurement of abnormal Red Blood Cell (RBC) deformability is a main indicator of Sickle Cell Anemia (SCA) and requires standardized quantification methods. Ektacytometry is commonly used to estimate the fraction of Sickled Cells (SCs) by...
IEEE/ACM transactions on computational biology and bioinformatics
Jan 1, 2021
Despite fluorescent cell-labelling being widely employed in biomedical studies, some of its drawbacks are inevitable, with unsuitable fluorescent probes or probes inducing a functional change being the main limitations. Consequently, the demand for a...
Quantitative phase microscopy (QPM) is a label-free technique that enables monitoring of morphological changes at the subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence propert...
SIGNIFICANCE: Label-free quantitative phase imaging is a promising technique for the automatic detection of abnormal red blood cells (RBCs) in real time. Although deep-learning techniques can accurately detect abnormal RBCs from quantitative phase im...
Computer methods in biomechanics and biomedical engineering
Nov 1, 2020
In order to have research on the deformation characteristics and mechanical properties of human red blood cells (RBCs), finite element models of RBC optical tweezers stretching and atomic force microscope (AFM) indentation were established. Non-linea...