AIMC Topic: Real-Time Polymerase Chain Reaction

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Development of a quantitative PCR for the detection of Rangelia vitalii.

Veterinary parasitology Dec 28, 2015
The aim of this study was to develop and validate a SYBR Green qPCR assay to detect and quantify a fragment of the 18S rRNA gene of Rangelia vitalii in canine blood. Repeatability of the qPCR was determined by the intra- and inter-assay variations. T...
Sensitivity and Specificity Animals RNA, Ribosomal, 18S Piroplasmida Protozoan Infections, Animal Veterinary Medicine Brazil Real-Time Polymerase Chain Reaction Dogs Dog Diseases
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Microarray expression profiling and gene ontology analysis of long non-coding RNAs in spontaneously hypertensive rats and their potential roles in the pathogenesis of hypertension.

Molecular medicine reports Nov 11, 2015
Long non-coding RNAs (lncRNAs) have been demonstrated to be significant in numerous biological processes. Hypertension is a form of cardiovascular disease with at least one billion cases worldwide. The present study sought to compare the differential...
Gene Expression Profiling Real-Time Polymerase Chain Reaction Hypertension RNA, Long Noncoding Gene Expression Regulation Reproducibility of Results Animals Rats, Inbred WKY Down-Regulation Gene Ontology Oligonucleotide Array Sequence Analysis Rats, Inbred SHR
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Survival and Metabolic Activity of Pediocin Producer Pediococcus acidilactici UL5: Its Impact on Intestinal Microbiota and Listeria monocytogenes in a Model of the Human Terminal Ileum.

Microbial ecology Jul 11, 2015
Pediococcus acidilactici UL5 is a promising probiotic candidate due to its high survival rate under gastric and duodenal conditions and to its ability to produce the antilisterial bacteriocin pediocin PA-1. Its survival, metabolic activity, and impac...
Probiotics Listeria monocytogenes Humans Ileum Real-Time Polymerase Chain Reaction Fermentation Pediocins Propidium Azides Pediococcus acidilactici Gastrointestinal Microbiome RNA, Ribosomal, 16S
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Cytotoxic response of platinum-coated gold nanorods in human breast cancer cells at very low exposure levels.

Environmental toxicology Apr 6, 2015
Because of unique optical behavior gold nanorods (GNRs) have attracted attention for the application in biomedical field such as bio-sensing, bio-imaging and hyperthermia. However, toxicological response of GNRs is controversial due to their differen...
Down-Regulation Female Up-Regulation Breast Neoplasms Nanotubes Real-Time Polymerase Chain Reaction Gold Reactive Oxygen Species Acetylcysteine RNA, Messenger Caspase 3 Membrane Potential, Mitochondrial Caspase 9 Apoptosis Particle Size Platinum Humans G1 Phase Cell Cycle Checkpoints MCF-7 Cells Proto-Oncogene Proteins c-bcl-2
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Printing 2-dimentional droplet array for single-cell reverse transcription quantitative PCR assay with a microfluidic robot.

Scientific reports Apr 1, 2015
This paper describes a nanoliter droplet array-based single-cell reverse transcription quantitative PCR (RT-qPCR) assay method for quantifying gene expression in individual cells. By sequentially printing nanoliter-scale droplets on microchip using a...
Single-Cell Analysis Cell Line MicroRNAs Robotics Humans Reproducibility of Results Real-Time Polymerase Chain Reaction Equipment Design Microfluidics
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Predicting fecal sources in waters with diverse pollution loads using general and molecular host-specific indicators and applying machine learning methods.

Journal of environmental management Jan 10, 2015
In this study we use a machine learning software (Ichnaea) to generate predictive models for water samples with different concentrations of fecal contamination (point source, moderate and low). We applied several MST methods (host-specific Bacteroide...
Artificial Intelligence Software Real-Time Polymerase Chain Reaction Feces Water Pollution Bacteria Water Microbiology Coliphages Environmental Monitoring
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Molecular classification of amyotrophic lateral sclerosis by unsupervised clustering of gene expression in motor cortex.

Neurobiology of disease Dec 10, 2014
Amyotrophic lateral sclerosis (ALS) is a rapidly progressive and ultimately fatal neurodegenerative disease, caused by the loss of motor neurons in the brain and spinal cord. Although 10% of ALS cases are familial (FALS), the majority are sporadic (S...
Motor Cortex RNA, Messenger Cluster Analysis Unsupervised Machine Learning Gene Expression Amyotrophic Lateral Sclerosis White People Immunohistochemistry Oligonucleotide Array Sequence Analysis Male Middle Aged Gene Expression Profiling Real-Time Polymerase Chain Reaction Humans Female Aged Adult
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Combining In Vivo Corneal Confocal Microscopy With Deep Learning-Based Analysis Reveals Sensory Nerve Fiber Loss in Acute Simian Immunodeficiency Virus Infection.

Cornea May 1, 2021
PURPOSE: To characterize corneal subbasal nerve plexus features of normal and simian immunodeficiency virus (SIV)-infected macaques by combining in vivo corneal confocal microscopy (IVCM) with automated assessments using deep learning-based methods c...
Humans Deep Learning Neural Networks, Computer Female Real-Time Polymerase Chain Reaction Microscopy, Confocal Disease Models, Animal Eye Infections, Viral Cornea Acute Disease Macaca nemestrina Simian Acquired Immunodeficiency Syndrome Animals RNA, Viral Simian Immunodeficiency Virus Nerve Fibers Male Trigeminal Nerve Diseases Macaca mulatta Middle Aged
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Effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on excisional wound healing in a rat's model.

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan Feb 1, 2019
OBJEVTIVE: To investigate the effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on the excisional wound healing in a rat's model.
Wound Healing Aconitum Real-Time Polymerase Chain Reaction Interleukin-10 Disease Models, Animal Fibroblast Growth Factor 2 Rats Tumor Necrosis Factor-alpha Pinellia Drugs, Chinese Herbal Interleukin-2 Interleukin-4 Transforming Growth Factor beta1 Rats, Sprague-Dawley Animals
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ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.

Nucleic acids research Jan 4, 2018
Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable ex...
Real-Time Polymerase Chain Reaction Mice Genes, Essential Knowledge Bases RNA, Untranslated Humans Animals Databases, Nucleic Acid Gene Expression Profiling
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