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Primary Cell Culture

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High Mobility Group Box 1 Protein Enhances HIV Replication in Newly Infected Primary T Cells.

Clinical laboratory
BACKGROUND: High-mobility group box 1 (HMGB1), a DNA-binding protein, has recently been shown to have effects on HIV replication, but the effects are dependent on the cell type and the timing of infection. Using human primary T cells, this study aime...

The effect of Intravenous Immunoglobulin (IVIG) on \textit{ex vivo} activation of human leukocytes.

Human antibodies
INTRODUCTION: Intravenous immunoglobulin (IVIG) has been widely used to treat various conditions, including inflammatory and autoimmune diseases. IVIG has been shown to have a direct influence on neutrophils, eosinophils and lymphocytes. However, man...

Quantifying co-cultured cell phenotypes in high-throughput using pixel-based classification.

Methods (San Diego, Calif.)
Biologists increasingly use co-culture systems in which two or more cell types are grown in cell culture together in order to better model cells' native microenvironments. Co-cultures are often required for cell survival or proliferation, or to maint...

A Systems Toxicology Approach for the Prediction of Kidney Toxicity and Its Mechanisms In Vitro.

Toxicological sciences : an official journal of the Society of Toxicology
The failure to predict kidney toxicity of new chemical entities early in the development process before they reach humans remains a critical issue. Here, we used primary human kidney cells and applied a systems biology approach that combines multidim...

AI-powered transmitted light microscopy for functional analysis of live cells.

Scientific reports
Transmitted light microscopy can readily visualize the morphology of living cells. Here, we introduce artificial-intelligence-powered transmitted light microscopy (AIM) for subcellular structure identification and labeling-free functional analysis of...

Machine learning identifies candidates for drug repurposing in Alzheimer's disease.

Nature communications
Clinical trials of novel therapeutics for Alzheimer's Disease (AD) have consumed a large amount of time and resources with largely negative results. Repurposing drugs already approved by the Food and Drug Administration (FDA) for another indication i...

Democratising deep learning for microscopy with ZeroCostDL4Mic.

Nature communications
Deep Learning (DL) methods are powerful analytical tools for microscopy and can outperform conventional image processing pipelines. Despite the enthusiasm and innovations fuelled by DL technology, the need to access powerful and compatible resources ...