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Reactive Oxygen Species

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Perfluorooctanoic acid-induced toxicity in primary cultures of chicken embryo cardiomyocytes.

Environmental toxicology
Perfluorooctanoic acid (PFOA) is a widespread environmental contaminant that induces developmental cardiotoxicity. It is detectable in late stage chicken embryos and hatchling chickens. To investigate mechanism(s) of cardiotoxicity, primary cultures ...

Cytotoxic response of platinum-coated gold nanorods in human breast cancer cells at very low exposure levels.

Environmental toxicology
Because of unique optical behavior gold nanorods (GNRs) have attracted attention for the application in biomedical field such as bio-sensing, bio-imaging and hyperthermia. However, toxicological response of GNRs is controversial due to their differen...

[Sensitization of human colon cancer HT-29 cells to TRAIL-induced apoptosis by gambognic acid].

Yao xue xue bao = Acta pharmaceutica Sinica
To investigate the effects of gambognic acid (GA) on TRAIL-induced apoptosis of cancer cells, human colon HT-29 cancer cells were treated with GA to promote apoptosis. Inhibition of the cell proliferation was measured with MTT assay and cell apoptosi...

Methodological considerations for using umu assay to assess photo-genotoxicity of engineered nanoparticles.

Mutation research. Genetic toxicology and environmental mutagenesis
In this study we investigated the feasibility of high-throughput (96-well plate) umu assay to test the genotoxic effect of TiO2 engineered nanoparticles (ENPs) under UV light (full spectrum) and visible light (455 nm). Exposure of TiO2 ENPs to up to ...

Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure.

Theriogenology
The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO2 NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. ...

Insulin improves in vitro survival of equine preantral follicles enclosed in ovarian tissue and reduces reactive oxygen species production after culture.

Theriogenology
This study investigated the effect of insulin concentration on the in vitro culture of equine preantral follicles enclosed in ovarian tissue. Ovarian tissue samples were immediately fixed (noncultured control) or cultured for 1 or 7 days in α-MEM(+) ...

Quantifying small molecule phenotypic effects using mitochondrial morpho-functional fingerprinting and machine learning.

Scientific reports
In primary fibroblasts from Leigh Syndrome (LS) patients, isolated mitochondrial complex I deficiency is associated with increased reactive oxygen species levels and mitochondrial morpho-functional changes. Empirical evidence suggests these aberratio...