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Cell Differentiation

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Basic fibroblast growth factor is critical to reprogramming buffalo (Bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells.

Theriogenology
Primordial germ cells (PGCs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (EG) cells in vitro. Buffalo PGC have been reported to be reprogrammed into EG-like cells, but the identities of the major...

Texture Descriptors Ensembles Enable Image-Based Classification of Maturation of Human Stem Cell-Derived Retinal Pigmented Epithelium.

PloS one
AIMS: A fast, non-invasive and observer-independent method to analyze the homogeneity and maturity of human pluripotent stem cell (hPSC) derived retinal pigment epithelial (RPE) cells is warranted to assess the suitability of hPSC-RPE cells for impla...

Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts.

BioMed research international
INTRODUCTION: Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation. Interleukin-1β is highly expressed during fracture healing and has been demonstrated to exert a significant...

Knowledge-Based Analysis for Detecting Key Signaling Events from Time-Series Phosphoproteomics Data.

PLoS computational biology
Cell signaling underlies transcription/epigenetic control of a vast majority of cell-fate decisions. A key goal in cell signaling studies is to identify the set of kinases that underlie key signaling events. In a typical phosphoproteomics study, phos...

Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.

Nature methods
Induced pluripotent stem cells (iPSCs) are an essential tool for modeling how causal genetic variants impact cellular function in disease, as well as an emerging source of tissue for regenerative medicine. The preparation of somatic cells, their repr...

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System.

Journal of visualized experiments : JoVE
Continued advancement in pluripotent stem cell culture is closing the gap between bench and bedside for using these cells in regenerative medicine, drug discovery and safety testing. In order to produce stem cell derived biopharmaceutics and cells fo...

What insights can spatiotemporal esophageal atlases and deep learning bring to engineering the esophageal mucosa?

Developmental cell
In this issue of Developmental Cell, Yang et al. present an integrated experimental and computational platform that maps the spatiotemporal development of the human esophagus and predicts key signaling pathways governing epithelial differentiation. T...

FateNet: an integration of dynamical systems and deep learning for cell fate prediction.

Bioinformatics (Oxford, England)
MOTIVATION: Understanding cellular decision-making, particularly its timing and impact on the biological system such as tissue health and function, is a fundamental challenge in biology and medicine. Existing methods for inferring fate decisions and ...

Deep Learning Method for Estimating Germ-layer Regions of Early Differentiated Human Induced Pluripotent Stem Cells on Micropattern Using Bright-field Microscopy Image.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Live cell staining is expensive and may bring potential safety issues in downstream clinical applications, bright-field images rather than staining images should be more suitable to reveal time-series changes of differentiating hiPSCs (human induced ...

Artificial Intelligence Supports Automated Characterization of Differentiated Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)
Revolutionary advances in AI and deep learning in recent years have resulted in an upsurge of papers exploring applications within the biomedical field. Within stem cell research, promising results have been reported from analyses of microscopy image...